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Application Notes
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Leuco-Malachite Green-NHS Ester is the labeling reagent precursor of malachite green: Once it is linked to a macro-molecule through reaction with a primary amino group followed by oxidation with reagents like DDQ, a malachite green moiety is generated. It can also be used as a precursor material for any malachite green derivative that involves a primary amino reaction. See below for oxidation and conversion methods.
Generation of Malachite Green from Leuco-Malachite Green:Excess DDQ (2,3-dichloro-5,6-dicyano quinone) is dissolved in DMSO or DMF, and added to the solution of leuco malachite green - target conjugate. The leuco-malachite green moiety is immediately oxidized to malachite green, which results in an intense green-blue coloration. The resulting malachite green - target conjugate can be purified by an appropriate method.
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