產(chǎn)品詳情

  • 產(chǎn)品名稱：LaminChromobody?plasmid(GFP)

  • 產(chǎn)品型號(hào)：
  • 產(chǎn)品廠商：ChromoTek
  • 產(chǎn)品文檔：
你添加了1件商品 查看購(gòu)物車
簡(jiǎn)單介紹：
LaminChromobody?plasmid(GFP)
詳情介紹：
Purpose With the Lamin-Chromobody?plasmid you can observe nuclear morphology in real time in your cell line of interest.
Brand Chromobody?
Specificity All Chromobody? binding domains are carefully selected to not interfere with endogenous protein function and offer the unique possibility to trace native proteins in living cells.
Characteristics Chromobodies? are a novel species of extremely small intracellular functional antibodies.

Chromobodies? are based on the antigen binding domain (VHH) derived from heavy chain antibodies of camelids genetically fused to a fluorescent protein. As opposed to conventional antibodies, these innovative fluorescent nanoprobes are suitable for real time analyses and can visualize endogenous cellular structures and processes in live cells.
Thus, Chromobodies? are the perfect reagents for cellular research including High-Content Analysis (HCA).

The Chromobody? plasmid allows you to create your own cell lines or transiently express the Chromobody?.
Background Antibodies - extremely powerful tools in biomedical research - are large complex molecules (150 kDa) consisting of two heavy and two light chains. Due to their complex structure the use of antibodies is often limited.

Camelidae (camels, dromedaries, llamas and alpacas) produce functional antibodies devoid of light chains, so called heavy chain antibodies (hcAbs). hcAbs recognize and bind their antigens via a single variable domain (VHH). These VHH domains are the smallest intact antigen binding fragments (molecular weight: 13 kDa), characterized by excellent stability, high specificity and affinities in the low nanomolar range.

The nuclear lamina is a meshwork enclosing the entire nucleus - the control center of eukaryotic cells. Its function is to hold together nuclear content and to add mechanical support to the nucleus. The nuclear lamina is a very sensitive subcellular structure. It has to maintain certain rigidity and at the same time allow structural dynamics. Therefore, artificial incorporation of overexpressed and fluorescently labeled lamin protein mostly leads to undesired cytotoxic side effects.
Application Notes Chromobodies? are a novel species of intracellular functional antibodies. Chromobodies? are made by genetically fusing the antigen binding domain (VHH) derived from heavy chain antibodies of camelids to fluorescent proteins, e.g. TagGFP2 or TagRFP.

With ChromoTek's unique Chromobody?-Technology the discrepancy and limitations of antibodies and fluorescent protein tagged reporters can now be overcome. You are now able to study the endogenous proteins of your target of interest in living cells - instead of artificial fluorescent fusion proteins which may behave differently from the real protein or may even be toxic to the cells. In combination with High-Throughput Microscopy and automated image analysis Chromobodies? set up a new class of intracellular biomarkers for High-Content Analysis (HCA). Our proof-of-principle studies include:
  • cell cycle monitoring
  • real time apoptosis studies
  • detection of posttranslational modification


These reagents thus significantly expand both the quality and the quantity of information that can be gathered in HCA.
Comment

The new Lamin-Chromobody? is a major breakthrough especially in High-Content Analysis (HCA) as apoptosis assays thus far rely on static endpoint assays.
The new Lamin-Chromobody? overcomes these unwanted side effects::
Visualize the nuclear lamina without interfering with its cellular functions.
Monitor the nuclear integrity and morphology during live cell microscopy as an indicator for cellular health and viability.
Use the Lamin-Chromobody? as biosensor for real-time assays of mitosis or apoptosis.
Protocol New way to trace nuclear morphology in live cells non-invasively!

With the Lamin-Chromoboy? plasmid you obtain the sequence of the Alpaca antibody to lamin fused to TagRFP or TagGFP. Upon transformation, your cells express the Lamin-Chromobody? and lamin is visualized by binding of the fluorescent Chromobody?. The transient binding does not influence lamin function and thus offers you the unique possibility to observe nuclear morphology in your cells.
Restrictions For Research Use only
Storage -80 °C
Storage Comment Store at -80°C. Short term storage at -20°C.