產(chǎn)品詳情

  • 產(chǎn)品名稱：Anti-MouseHRPAECDetectionSystem

  • 產(chǎn)品型號：
  • 產(chǎn)品廠商：SpringBio
  • 產(chǎn)品文檔：
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簡單介紹：
Anti-MouseHRPAECDetectionSystem
詳情介紹：
Characteristics The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated mouse primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme- labeled streptavidin, and substrate-chromogen.
Comment

The AEC end product is soluble in organic solvents and must be used with an aqueous counterstain and mounting media.
Protocol 1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
2. Add enough drops of Hydrogen Peroxide Block to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer.
3. Perform appropriate pretreatment if required. Wash 3 times in buffer.
4. Apply Protein Block (if required) and incubate for 10 minutes at room temperature to block nonspecific background staining. Wash 1 time in buffer.
5. Apply primary antibody and incubate according to manufacturer’s protocol.
6. Wash 4 times in buffer. Apply Biotinylated Goat Anti-Mouse and incubate for 10 minutes at room temperature. Wash 4 times in buffer.
7. Apply Streptavidin Peroxidase and incubate for 10 minutes at room temperature.
8. Rinse 4 times in buffer. Apply AEC Single Solution to tissue. Incubate for 10 minutes.
9. Rinse 4 times in buffer. Apply counterstain according to manufacturer’s instructions. 10. Apply a sufficient volume of an aqueous mounting medium to cover the section.
Restrictions For Research Use only
Format Liquid
Precaution of Use 1. Take reasonable precautions when handling reagents. Wear appropriate Personal Protective Equipment.
2. Avoid contact of reagents with eyes, mucous membranes and skin. If reagents come into contact with sensitive areas, wash with copious amounts of water.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment The user must validate any other storage conditions. The reagents must be returned to the storage conditions identified above immediately after use. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.