產(chǎn)品詳情

  • 產(chǎn)品名稱：StreptavidinAlkalinePhosphatase

  • 產(chǎn)品型號：
  • 產(chǎn)品廠商：SpringBio
  • 產(chǎn)品文檔：
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簡單介紹：
StreptavidinAlkalinePhosphatase
詳情介紹：
Characteristics This reagent is intended to be used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme- labeled streptavidin, and substrate-chromogen.
Protocol 1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
2. Perform appropriate pretreatment and blocking if required. Wash 3 times in buffer.
3. Apply primary antibody and incubate according to manufacturer’s protocol.
4. Wash 4 times in buffer. Apply appropriate biotinylated secondary antibody and incubate according to manufacturer’s protocol. Wash 4 times in buffer.
5. Apply Streptavidin Alkaline Phosphatase and incubate for 10 minutes at room temperature.
6. Rinse 4 times in buffer. Apply chromogen and substrate according to manufacturer’s instructions.
7. Apply counterstain according to manufacturer’s instructions.
8. Dehydrate and coverslip or use appropriate mounting medium.
Restrictions For Research Use only
Format Liquid
Buffer This product contains streptavidin alkaline phosphatase conjugate in PBS, pH 7.6.
Precaution of Use 1. Take reasonable precautions when handling reagents. Wear appropriate Personal Protective Equipment.
2. Avoid contact of reagents with eyes, mucous membranes and skin. If reagents come into contact with sensitive areas, wash with copious amounts of water.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment The user must validate any other storage conditions. The reagents must be returned to the storage conditions identified above immediately after use. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.