Characteristics
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Normal Goat IgG Control is essential for ELISA, WB, IHC and IP experiments. Their purpose is to verify that the proteins revealed in the experiment result from the specific interaction of the antibody with the antigen protein. Some people do use specific primary antibody alone but this does not consider of those proteins that may bind to regions of the IgG distinct from the antigen s. The best control is to use isotype-matched IgGfrom the same species (Goat) for the experiment. That is an IgG control should have the same immunoglobulin type and be used at the same concentration as the specific detection antibody.
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