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Format
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Lyophilized
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Reconstitution
|
Reconstitute in pure water
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Buffer
|
Modified RIPA Lysis Buffer: 50 mM Tris-HCl?pH 7.4, 150 mM NaCl, 1?mM EDTA, 1?% Triton X-100, 0.1?% SDS, 1?% Sodium deoxycholate, 1?mM PMSF.
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Handling Advice
|
Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
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Storage
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4 °C/-80 °C
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Storage Comment
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Store at 4°C. After re-dissolution, aliquot and store at -80°C.
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Expiry Date
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12 months
|
