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Isotype & Other Controls(同型對(duì)照,其他對(duì)照)
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產(chǎn)品名稱:GuineaPigIgGIsotypeControl
產(chǎn)品型號(hào):
產(chǎn)品廠商:BioLeaf
產(chǎn)品文檔:
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簡(jiǎn)單介紹:
GuineaPigIgGIsotypeControl
詳情介紹:
Product details
Product details
Isotype
IgG
Characteristics
Identity and purity is confirmed by immunoelectrophoresis and double radial immunodiffusion (Ouchterlony) using antisera to guinea pig total serum proteins.
Purification
The protein is isolated from plasma using ion exchange chromatography, affinity chromatography and gelfiltration. Chromatographically purified guinea pig IgG
Purity
> = 95 % as determined by agar-gel electrophoresis. Tested in a concentration of 10 mg/mL at the level of sensitivity of the applied immunoprecipitating techniques, this product contains traces of other proteins.
Target details
Target details
Research Area
Immunology, Secondary Antibodies
Application Details
Application Details
Application Notes
Purified guinea pig IgG may be used as a reference antigen, calibrator, coating protein and blocking agent in a variety of immunoassays including immunodiffusion, immunoelectrophoresis, ELISA, Western blotting, dot-immunobinding assays (DIBA), haemagglutination, and cell-binding assays.
Comment
Lyophilized, stable white powder.
Restrictions
For Research Use only
Handling
Handling
Format
Lyophilized
Reconstitution
After opening the vial, the lyophilized content is reconstituted by adding 1 mL of sterile distilled water, mixed gently by inversion until complete dissolution is obtained. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium.
Preservative
Without preservative
Handling Advice
Working dilutions may be prepared by adding the required amount of phosphate buffered saline (PBS), should not be refrozen and preferably used within 24 hours. Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product.
Storage
4 °C/-20 °C
Storage Comment
This product is shipped lyophilized at ambient temperature. After arrival store at +4 °C. Prolonged storage may be at -20 °C. Reconstituted solution may be stored frozen.