產(chǎn)品詳情

  • 產(chǎn)品名稱：RabbitIgGisotypecontrol(PE)

  • 產(chǎn)品型號(hào)：
  • 產(chǎn)品廠商：BioLeaf
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簡(jiǎn)單介紹：
RabbitIgGisotypecontrol(PE)
詳情介紹：
Isotype IgG
Characteristics This polyclonal antibody can be used as a negative control for immunofluorescence staining. This enables an estimation of non-specific binding of rabbit polyclonal antibodies to cell surface components in peripheral blood and tissue. Suitable for whole blood, Ficoll-separated preparations, frozen and paraffin embedded sections.
Purification Protein G purified.
Research Area Immunology, Secondary Antibodies
Application Notes It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 10 μL/10^6 cells. For use as an isotype control for direct immuno-fluoresence when using Phycoerythrin conjugated rabbit polyclonal antibodies. Suitable for whole blood, Ficoll-separated preparations, frozen and paraffin embedded tissue sections. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. We recommend using this reagent at the same concentration as the test reagent.It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 10 μL/10^6 cells.
Comment

R-Phycoerythrin (Europa Bioproducts, Ely, Cambridge).
Reagent Preparation
  1. Transfer 100 μL of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 10 μL of Rabbit Polyclonal IgG - Isotype Control PE and mix gently with a vortex mixer. The 10 μL is a guideline only, the correct volume depends on the quantity of direct monoclonal used. 3. Incubate in the dark at room temperature at 4 °C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 4. Add 1,5 mL of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 5. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 μL of fluid. 6. Add 2 mL 0.01 mol/L PBS (It betters that it containing 2 % bovine serum albumin) and resuspend the cells by using a vortex mixer. 7. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 μL of fluid. 8. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS. The PBS should contain 1 % paraformaldehyde (fixative) if samples are not analysed the same day. 9. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis.
Restrictions For Research Use only
Preservative Sodium azide
Precaution of Use 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
Handling Advice Product is photosensitive and should be protected from light.
Storage 4 °C