產(chǎn)品詳情

  • 產(chǎn)品名稱：alamarBlue?

  • 產(chǎn)品型號：
  • 產(chǎn)品廠商：Bio-Rad
  • 產(chǎn)品文檔：
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簡單介紹：
alamarBlue?
詳情介紹：
Purpose The cell proliferation assay reagent alamarBlue? is designed to provide a rapid and sensitive measure of cell proliferation and cytotoxicity in various human and animal cell lines, bacteria and fungi.
Characteristics alamarBlue? is an indicator dye, that incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and changes colour in response to the chemical reduction of growth medium, resulting from cell growth. The alamarBlue? cell proliferation assay reagent is designed to quantitatively measure the proliferation of various human and animal cell lines, bacteria and fungi.
Application Notes - Cell proliferation assays.
- The reagent can be used to establish proliferation or relative cytotoxicity in a cell proliferation assay.
- Baseline data for predicting the toxicity of related novel agents can be compared to baseline data with known in-vivo toxicity.
- alamarBlue? is for use between pH6.8 and pH7.4.
Protocol Cell proliferation assay.
- Growing cells cause a chemical reduction of alamarBlue?.
- Continued growth maintains a reduced environment. (fluorescent, red).
- Inhibit on of growth maintains an oxidized environment. (non-fluorescent, blue).
- Data may be collected using either fluorescence-based or absorbance-based instrumentation.
- Fluorescence is monitored at 530-560nm excitation wavelength and 590nm emission wavelength.
- Absorbance is monitored at 570nm and 600nm.
Restrictions For Research Use only
Format Liquid
Handling Advice This product is photosensitive and should be protected from light.
Storage 4 °C
Storage Comment This product may be stored for 12 months at room temperature. Store at 2-8°C to increase the shelf life to 20 months. This product should be stored undiluted.