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Characteristics
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Reaction of the alkyne group with the azide group is typically accomplished by copper-catalyzed Click Chemistry. Within physiologically-relevant temperature and pH ranges, IRDye 800CW Alkyne is inert toward naturally-occurring nucleophiles such as amines and hydroxyls. At higher pH or upon exposure to short-wave ultraviolet (UV) light, the alkyne group may become unstable toward sulfhydryls (-SH, thiols), dihalogens such as iodine (I2), and organophosphines such as tris(2-carboxylethyl)phosphine (TCEP).
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