產(chǎn)品詳情

  • 產(chǎn)品名稱：Caspase-4,5,9FluorogenicSubstrate

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  • 產(chǎn)品廠商：KamiyaBiomedical
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Caspase-4,5,9FluorogenicSubstrate
詳情介紹：
Purpose Dissolve the caspase inhibitor in high purity DMSO (>99.9%) before use. Fluorometer calibration: The fluorometer is calibrated using known concentrations of free AFC (Excitation = 400 nm, Emission = 505 nm) to generate a calibration curve of fluorescence versus μ-moles AFC. Samples: Can be either purified or partially purified caspase preparations. Crude cell lysates can be assayed if the non-specific protease background is determined using our Caspase-4, -5, -9 Inhibitor (Z- LEHD-FMK).
Sequence Acetyl-Leu-Glu-His-Asp-AFC-FA, Ac-LEHD-AFC
Specificity Serves equally well as a substrate for Caspases-4, -5, and -9. Can also serve as a weak substrate for Caspases-1, -2, -6, and -8 at an efficiency of 30 - 40% .
Purity > 95 % by HPLC
Background Trifluoroacetic acid salt of a peptide substrate labeled at the carboxy end with AFC (7-amino-4- trifluoromethyl coumarin). Designed to measure Caspase-4, Caspase-5, or Caspase-9 activity in vitro. Caspase-4 (also known as ICErel-II, TX, or ICH-2), Caspase-5 (also known as ICErel-III or TY), and Caspase-9 (also known as ICE-LAP6 or Mch6) are members of the caspase family of cysteine proteases involved in apoptosis. Caspases-4 and - 5 belong to Group I (along with caspase-1), which prefer the tetrapeptide substrate sequence WEHD and are thought to be involved in inflammation through the maturation of pro-IL-1betaa. Their role in apoptosis, however, is unclear. Caspase-9 is a member of Group III, which prefer the substrate sequence (L/V)jEXD. Since Caspase-9 has a strict requirement for His in the P4 position, it is not unexpected that the LEHD inhibitor sequence would work well on this caspase. The Group III caspases optimal recognition sequence resembles the activation sites within several effector caspase proenzymes, implicating the Group III enzymes as upstream components in the proteolytic cascade that amplifies the death signal.
Molecular Weight 765 Da (not including TFA salt). 879 Da (including TFA salt).
Application Notes For in vitro assays of Caspases-4, -5, and -9 activity. Can be used with purified or partially purified enzymes, or possibly with crude cell lysates (if the Caspase-4, -5, -9 Inhibitor is included to determine background protease activity).
Restrictions For Research Use only
Format Solid
Storage RT