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  • 產(chǎn)品名稱：Caspase-1,4,5Substrate-pNA

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  • 產(chǎn)品廠商：KamiyaBiomedical
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Caspase-1,4,5Substrate-pNA
詳情介紹：
Purpose Soluble in DMSO and aqueous buffers. We recommend preparing a stock solution in DMSO, and diluting into aqueous buffer shortly prior to use.
1. Lyse cells in 50 mM Tris-HCl, pH 7.5, 0.3% NP-40, 1.0 mM DTT, at a density of 2 X 10^6 /mL.
2. Assay 0.01 ml cell lysate in a final volume of 0.1 ml. Assay buffer is cell lysis buffer containing 0.2 mM substrate.
3. Incubate at 37° C for 0-3 hr. Take periodic readings of absorbance at 405 nm.
Sequence Acetyl-Trp-Glu-His-Asp-pNA.TFA, Ac-WEHD-pNA
Specificity Substrate for caspase-1, caspase-4, and caspase-5.
Purity > 97 % by HPLC
Background TFA salt of a paranitroanilide- peptide substrate for caspases-1, -4, and -5. Release of free pNA is monitored by absorbance at 405 nm (epsilon=9,160 M^-1 cm^-1 ). Caspase-1 (also known as ICE), Caspase-4 (also known as ICErel-II, TX, or ICH-2), and Caspase-5 (also known as ICErel-III or TY) make up the Group I caspases, all of which prefer the tetrapeptide substrate sequence WEHD. Although Group I caspases are involved in inflammation through the maturation of pro-IL- 1beta. There is evidence suggesting that activation of Group I caspases induces apoptosis, although substrate specificity studies do not provide compelling evidence for a role of Group I caspases in apoptosis since hydrophobic amino acids in the P4 position (prefered by Group I) are not observed in proteins cleaved during apoptosis. Most research supports a role for Caspase-1 in inflammation but the roles of Caspase-4 and Caspase-5 have not been established.
Molecular Weight 747 Da
Application Notes Assay of caspase activity in cell extracts.
Comment

Formula: C34H37N9O11
Restrictions For Research Use only
Format Lyophilized
Storage RT
Background publications Talanian, Quinlan, Trautz, Hackett, Mankovich, Banach, Ghayur, Brady, Wong: "Substrate specificities of caspase family proteases." in: The Journal of biological chemistry, Vol. 272, Issue 15, pp. 9677-82, 1997 (PubMed).

Thornberry, Rano, Peterson, Rasper, Timkey, Garcia-Calvo, Houtzager, Nordstrom, Roy, Vaillancourt, Chapman, Nicholson: "A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 29, pp. 17907-11, 1997 (PubMed).

Faucheu, Diu, Chan, Blanchet, Miossec, Hervé, Collard-Dutilleul, Gu, Aldape, Lippke: "A novel human protease similar to the interleukin-1 beta converting enzyme induces apoptosis in transfected cells." in: The EMBO journal, Vol. 14, Issue 9, pp. 1914-22, 1995 (PubMed).

Kamens, Paskind, Hugunin, Talanian, Allen, Banach, Bump, Hackett, Johnston, Li: "Identification and characterization of ICH-2, a novel member of the interleukin-1 beta-converting enzyme family of cysteine proteases." in: The Journal of biological chemistry, Vol. 270, Issue 25, pp. 15250-6, 1995 (PubMed).

Munday, Vaillancourt, Ali, Casano, Miller, Molineaux, Yamin, Yu, Nicholson: "Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases." in: The Journal of biological chemistry, Vol. 270, Issue 26, pp. 15870-6, 1995 (PubMed).