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  • 產(chǎn)品名稱：MalachiteGreen-NHSEster

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MalachiteGreen-NHSEster
詳情介紹：
Characteristics Molecular reporter quenching molecule with NHS Ester conjugation group.
Purity 30 %
Background Malachite Green-NHS Ester (MG-quenchTM ) is a technical grade fluorescent quenching labeling reagent. It can be linked to any molecule with a primary amino group, and it quenches fluorescence from molecules such as fluorescein derivatives. This water soluble molecule enables its wide applicability in labeling target molecules in biological systems. It can also serve as an alternative choice for the same role of Dabcyl, or Black hole quencher (BHQ) derivatives as a quenching moiety for applications such as molecular beacons and probes. Below is the reaction scheme between Malachite Green-NHS Ester and free amino groups in oligonucleotides or peptides.
Molecular Weight 698.55 Da
Application Notes Example Protocol to Conjugate Malachine Green-NHS Ester to Oligonucleotides. (The same methods apply for conjugation of Leuco-Malachite Green NHS Ester to amines).The succinimide ester of Malachite Green (21 mg, 30.11 micromol) is dissolved in 1.0 mL methyl sulfoxide. The amino-oligo is then dissolved in 800 microL of 0.2 M sodium carbonate buffer (pH 9.5). The ester solution (400 microL) is added to the amino-oligo solution. The mixture is then left for 16 hour at 4 degrees C, after which, it is chromatographed on a Sephadex G-25 column using deionized water/carbonate buffer (50/50) as eluent. The fraction with deep blue coloration is dialyzed against water to remove excess salt and unreacted reagents, and then freeze-dried. The final product is stored in the refrigerator until use.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Solubility: Soluble in Water
Storage 4 °C
Supplier Images
image for Malachite Green-NHS Ester (ABIN1059082) Malachite Green-NHS Ester