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  • 產(chǎn)品名稱：Rabbitanti-HumanIgA(HeavyChain),(Chainalpha)Antibody(Biotin)

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  • 產(chǎn)品廠商：Acris-antibodies
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簡單介紹：
Rabbitanti-HumanIgA(HeavyChain),(Chainalpha)Antibody(Biotin)
詳情介紹：
Immunogen Human IgA alpha heavy chain
Characteristics Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule
Purification Immunoaffinity Chromatography.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Background Synonyms: Human Immunoglobulin A
Research Area Immunology, Secondary Antibodies
Application Notes Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Recommended Dilutions: This product has been assayed against 1.0?μg of Human IgA in astandard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30?minutes atroom temperature. A working dilution of 1:10,000 to 1:100,000 of the reconstitutionconcentration is suggested for this product.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 1.0?mL of deionized water or equivalent.
Concentration 1.0 mg/mL
Buffer 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride,?pH 7.4, containing 10?mg/mL BSA (IgG and Protease free) and 0.01?% (w/v) Thimerisol as preservative
Preservative Thimerosal (Merthiolate)
Precaution of Use Biohazard Informations: This product contains thimerosal which is hazardous.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use.
Centrifuge product if not completely clear after standing at room temperature.
Storage 4 °C/-20 °C
Storage Comment Store vial at 2-8 °C prior to restoration. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage reconstitute product with 50 % glycerol instead of water and then aliquot contents and freeze at -28 °C or below.
Background publications Bayer, Wilchek: "Protein biotinylation." in: Methods in enzymology, Vol. 184, pp. 138-60, 1990 (PubMed).